A rapid quantitative method for the separation of free fatty acids from other lipids

Some new methods for separation and analysis of fatty acids and other lipids.

Rapid colorimetric micromethod for free fatty acids.

Free fatty acids (FFA), the uranyl ion, and the basic dye Rhodamine B form colored complexes, which are extractable into toluene or benzene. Fatty acids of different chain lengths above C(10) and different degrees of unsaturation gave constant molar yield. Complexes in toluene alone are unstable, especially in the light, but a small amount of aqueous uranyl acetate stabilizes them sufficiently ...

"a gas chromatographic method for the determination of individual free fatty acids in serum"

serum fatty acids on 30 healthy lranian women were detemined by a gas chromatographic method. oleic and stearic acids were used as standards. the mean values were myristic acid 5. 7% , palmitic acid 43% , palmitoleic acid 5.3% , stearic acid 8% , oleic acid 21. 3% and linleic acid 16.4% of total fatty acid serum contents.

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.

Quantitative release of fatty acids from lipids by a simple hydrolysis procedure.

Glycerolipids and sphingolipids are hydrolyzed with 0.5 M HCl in acetonitrile-water 9:1 (by vol) for 45 min at 100 degrees C or 4 hr at 70 degrees C. After hydrolysis, free fatty acids (FFA) are recovered in chloroform and separated by thin-layer chromatography. More than 95% of the radioactivity from labeled phospholipids is recovered as FFA, and more than 97% of the lipid phosphorus is recove...